The gall midge Contarinia petioli has a larva that causes a gall to form on the petiole (stalk) of Aspen (Populus tremula) leaves. The gall is seen more often than the midge. The gall is globular or pear shaped, 3 to 8 mm across and sometimes reddens. It can occasionally occur in the stem at the junction with a petiole, and also it seems where the petiole enters the leaf. The galls contain one, or a few chambers, each with one orange larva; a circular opening develops in mature gall through which larva leave.
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